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1IntroductionCadmiumisoneofimportantenvironmentalpolutants.Itisverytoxictobiology(Barber,1994;Colard,1990;Goyer,1995;Nassiri,... 相似文献
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水环境中耐热大肠菌群的抗生素耐药性与质粒谱研究 总被引:5,自引:0,他引:5
用滤膜法、mFC培养基从5种水体中分离出疑似耐热大肠菌162株,用API微生物分析系统鉴定到种,以Kirby-Bauer法分析其对人畜常用10种抗生素的耐药性,碱裂解法小量制备各菌株质粒DNA做质粒谱分析.结果表明,埃希氏大肠杆菌为优势菌,占分离菌总数的96.3%.除分离自泉水的3株外,其它菌株都对3种及3种以上抗生素耐药,多重耐药率为98.1%.不同水体分离菌株对氧氟沙星、诺氟沙星、庆大霉素、丁胺卡那霉素、链霉素的耐药率有显著性差异(P<0.005).92株菌(56.8%)提取到大小为0.90~158.83kb、数量为1~6个的质粒,有81种质粒谱型.70株(43.2%)未提取到质粒的细菌中有67株为多重耐药.具有相同质粒谱型的菌株耐药谱都不相同.未发现质粒谱与抗生素耐药性间有明显相关性.图1表3参15 相似文献
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Vijayalakshmi Pradeep 《Journal of environmental science and health. Part. B》2013,48(5):346-360
The present study was undertaken with the objective of studying repeated batch and continuous degradation of chlorpyrifos (O,O-diethyl O-3,5,6-trichloropyridin-2-yl phosphorothioate) using Ca-alginate immobilized cells of Pseudomonas putida isolated from an agricultural soil, and to study the genes and enzymes involved in degradation. The study was carried out to reduce the toxicity of chlorpyrifos by degrading it to less toxic metabolites. Long-term stability of pesticide degradation was studied during repeated batch degradation of chlorpyrifos, which was carried out over a period of 50 days. Immobilized cells were able to show 65% degradation of chlorpyrifos at the end of the 50th cycle with a cell leakage of 112 × 103 cfu mL?1. During continuous treatment, 100% degradation was observed at 100 mL h?1 flow rate with 2% chlorpyrifos, and with 10% concentration of chlorpyrifos 98% and 80% degradation was recorded at 20 mL h?1 and 100 mL h?1 flow rate respectively. The products of degradation detected by liquid chromatography–mass spectrometry analysis were 3,5,6-trichloro-2-pyridinol and chlorpyrifos oxon. Plasmid curing experiments with ethidium bromide indicated that genes responsible for the degradation of chlorpyrifos are present on the chromosome and not on the plasmid. The results of Polymerase chain reaction indicate that a ~890-bp product expected for mpd gene was present in Ps. putida. Enzymatic degradation studies indicated that the enzymes involved in the degradation of chlorpyrifos are membrane-bound. The study indicates that immobilized cells of Ps. putida have the potential to be used in bioremediation of water contaminated with chlorpyrifos. 相似文献
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Molecular characterization of soil bacteria antagonistic to Rhizoctonia solani,sheath blight of rice
Jasdeep C. Padaria Aqbal Singh 《Journal of environmental science and health. Part. B》2013,48(4):397-402
Bacillus pumillus MTCC7615 has been identified as a potent isolate against Rhizocotonia solani, the fungal pathogen causing sheath blight in rice. The study aimed at probing the role of a 23kb size plasmid pJCP07 of Bacillus pumillus MTCC7615 in its fungal antagonism towards Rhizocotonia solani. Plasmid pJCP07 was found to be involved in production of a fungal antagonistic compound as demonstrated by plasmid curing and conjugational transfer experiments. Tn5 insertional studies further confirmed that the plasmid pJCP07 of Bacillus pumillus MTCC7615 carries some of the gene(s) involved in production of compound antagonistic to Rhizocotonia solani. The plasmid pJCP07 is thus a mobilizable medium-sized plasmid carrying genes responsible for antagonism of Bacillus pumillus MTCC7615 towards Rhizocotonia solani. 相似文献
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萘降解质粒pND6的分离和鉴定 总被引:1,自引:1,他引:0
从工业废水中分离的假单胞菌 (Pseudomonassp .)ND6菌株 ,能以萘为唯一碳源生长 ,使无机盐培养基(MM)中 2g/L的萘在 48h内降解 98% .该菌株含有一个 115kb的大质粒pND6 .DNA杂交实验表明 ,pND6质粒含有与恶臭假单胞菌 (P .putida)G7菌株的NAH7质粒同源的萘降解基因 .图 3表 1参 14 相似文献
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发根农杆菌转化青蒿影响因素的研究 总被引:7,自引:0,他引:7
对叶盘法转化过程中各种影响因素进行研究.结果表明:发根农杆菌种类、基本培养基和青蒿株系对转化率有明显影响,而培养基pH在5.2~5.8对转化率没有明显影响;发根农杆菌ATCC15834的转化率最高;基本培养基中MS效果最好;青蒿株系025对发根农杆菌最敏感;处于对数生长期的发根农杆菌(D660nm=0.75)稀释5倍后用来转化效果最好;幼嫩的叶片的转化率比成熟叶片高;向共培养培养基中加入乙酰丁香酮等酚类物质对转化没有促进作用.在最适的转化条件下,发根农杆菌ATCC15834对青蒿株系025的转化率可达到100%.PCR检测证明,青蒿发根基因组中含有发根农杆菌Ri质粒TDNA上的rolC片段. 相似文献